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008			081221s2003 gw a |b 001 0 eng
020			_a3527304657 (alk. paper)
039		9	_a201308051227 _bmalmash _c201308051128 _dmalmash _c201006291232 _dmalmash _c200812230839 _dvenkatrajand _y200812210901 _zNoora
050	0	0	_aRS403 _b.B54 2003
245	0	0	_aBioNMR in Drug Research / _cedited by Oliver Zerbe.
260			_aWeinheim : _bWiley-VCH, _cc2003.
300			_axxviii, 484 p. : _bill.
440		0	_aMethods and principles in medicinal chemistry ; _vv. 16 _918492
504			_aIncludes bibliographical references and index.
505			_aPreface.Foreword.List of Authors.List of Abbreviations.PART I: BASIC TECHNIQUES.Modern Methods for the Expression of Proteins in Isotopically Enriched Form (H. Patzelt, et al.).Structure Calculation Using Automated Techniques (P. Guntert).Achieving Better Sensitivity, Less Noise and Fewer Artifacts in NMR Spectra (D. Moskau & O. Zerbe).PART II: NMR OF BIOMOLECULES.NMR Strategies for Protein Assignments (V. Dotsch).NMR of Membrane-Associated Peptides and Proteins (R. Bader, et al.).NMR of Nucleic Acids (R. Fiala & V. Sklenar). PART III MODERN SPECTROSCOPIC TECHNIQUES.Methods for the Measurement of Angle Restraints from Scalar, Dipolar Couplings from Cross-Correlated Relaxation: Application to Biomacromolecules (C. Griesinger).Orientational Restraints (E. de Alba & N. Tjandra).Scalar Coupling Across Hydrogen Bonds (A. Dingley, et al.).TROSY: Transverse Relaxation-Optimized Spectroscopy (R. Riek).MAS Solid-State NMR of Isotopically Enriched Biological Samples (P. Williamson , et al.).Determination of Protein Dynamics Using 15N Relaxation Measurements (D. Fushman). PART IV: TOOLS FOR INVESTIGATION OF DRUG - RECEPTOR COMPLEXES AND FOR LIGAND SCREENING.The Determination of Equilibrium Dissociation Constants of Protein-Ligand Complexes by NMR (G. Roberts).Experiments in NMR-Based Screening (C. Marchioro, et al.).The Use of Spin Labels in NMR-Supported Lead Finding and Optimization (W. Jahnke).NMR of Weakly Binding Ligands (M. Blommers & S. Rudisser).Isotope Filter and Editing Techniques (G. Gemmecker). PART V: STRATEGIES FOR DRUG DEVELOPMENT USING NMR.Strategies for NMR Screening and Library Design (C. Lepre).Strategies for Hit Finding Using NMR (W. Klaus & H. Senn).Strategies for Drug Discovery Using NMR (M. Blommers, et al.).NMR-Based Drug Design: Approaches for Very Large Proteins (M. Pellecchia, et al.).Subject Index.
520			_aThe vast progress made in the investigation of biomolecules using NMR has only recently been rewarded with the Nobel Prize for Kurt Wuthrich. Edited by a former coworker of Wuthrich, this book presents the theoretical background on NMR of biomolecules, plus the use of NMR techniques in determining the structures of proteins and nucleic acids. BioNMR spectroscopy offers a universal tool for examining the binding of an active substance to its target protein. Its use thereby benefits the rational development of drugs. This interaction can now be investigated in a hitherto unparalleled precision and displayed in 3D an important prerequisite for the targeted development of new active substances. The latest methods for characterizing substance receptor complexes are demonstrated backed by many case studies from pharmaceutical research. Thus it comes as no surprise that a large number of the authors are working for leading pharmaceutical companies. With its successful mixture of basic information and application strategies, coupled with many real life examples, this is an invaluable guide for both NMR spectroscopists and pharmaceutical researchers.
650		0	_aPharmaceutical chemistry. _918493
650		0	_aNuclear magnetic resonance spectroscopy. _918494
650	1	2	_aDrug Design. _918495
650	2	2	_aChemistry, Pharmaceutical _xmethods. _918496
650	2	2	_aMagnetic Resonance Spectroscopy _xmethods. _918497
650	2	2	_aNuclear Magnetic Resonance, Biomolecular _xmethods. _918498
650	2	2	_aProtein Conformation. _918499
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999			_c7178 _d7178