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020 _a0471986623 (cloth : acidfree paper)
020 _a0471986631 (paper back : acid-free paper)
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050 0 0 _aQD476.2
_b.S42 2000
100 1 _aSheehan, David.
_918486
245 1 0 _aPhysical Biochemistry :
_bPrinciples and Applications /
_cDavid Sheehan.
260 _aChichester ;
_aNew York :
_bWiley,
_cc2000.
300 _axvi, 349 p. :
_bill. ;
_c26 cm.
504 _aIncludes bibliographical references and index.
505 _aPreface Acknowledgements Chapter 1 Introduction 1.1 Special chemical requirements of biomolecules 1.2 Factors affecting analyte structure and stability 1.2.1 pH effects 1.2.2 Temperature effects 1.2.3 Effects of solvent polarity 1.3 Buffering systems used in biochemistry 1.3.1 How does a buffer work? 1.3.2 Some common buffers 1.3.3 Additional components often used in buffers 1.4 Quantitation, units and data handling 1.4.1 Units used in this text 1.4.2 Quantitation of protein and biological activity 1.5 Objectives of this book Bibliography Chapter 2 Chromatography 2.1 Principles of chromatography 2.1.1 The partition coefficient 2.1.2 Phase systems used in biochemistry 2.1.3 Liquid chromatography 2.1.4 Gas chromatography 2.2 Performance parameters used in chromatography 2.2.1 Retention 2.2.2 Resolution 2.2.3 Physical basis of peak broadening 2.2.4 Plate height equation 2.2.5 Capacity factor 2.2.5 Peak symmetry 2.2.7 Significance of performance criteria in chromatography 2.3 Chromatography equipment 2.3.1 Outline of standard system used 2.3.2 Components of a chromatography system 2.3.3 Stationary phases used 2.3.4 Elution 2.4 Modes of chromatography 2.4.1 Ion exchange 2.4.2 Gel filtration 2.4.3 Reversed phase 2.4.4 Hydrophobic interaction 2.4.5 Affinity 2.4.6 Immobilised metal affinity chromatography 2.4.7 Hydroxyapatite 2.5 Open-column chromatography 2.5.1 Equipment used 2.5.2 Industrial-scale chromatography of proteins 2.6 High-performance liquid chromatography 2.6.1 Equipment used 2.6.2 Stationary phases in HPLC 2.6.3 Liquid phases in HPLC 2.7 Fast protein liquid chromatography 2.7.1 Equipment used 2.7.2 Comparison with HPLC 2.8 Perfusion chromatography 2.8.1 Theory of perfusion chromatography 2.8.2 The practice of perfusion chromatography 2.9 Membrane-based chromatography systems 2.9.1 Theoretical basis 2.9.2 Applications of membrane-based separations 2.10 Chromatography of a sample protein 2.10.1 Desig
520 _aAn accessible and easy-to-read text for students requiring a broad overview of the key techniques used to characterise the structure and function of complex biomacromolecules such as proteins and DNA. It bridges the gap between general biochemistry textbooks and the more specialist texts covering individual techniques. Topics covered include chromatography, spectroscopy, mass spectrometry, electrophoresis, X-ray diffraction, centrifugation and biocalorimetry. New developments are placed in context by describing the physical principles on which they depend, examining the range of biophysical applications most widely used and, emphasising the overall similarities of experimental approach. Written by a biochemist with extensive teaching experience, the book: describes the advantages and disadvantages of each technique and compares one technique to another; introduces experimental approaches in a non-mathematical way, using practical examples; and provides a bibliography, including useful web sites, at the end of each chapter. This book will be invaluable to undergraduates and postgraduates studying biochemistry, molecular biology and related disciplines.
650 0 _aPhysical biochemistry.
_91719
942 _2lcc
_n0
_cBK
999 _c7173
_d7173