Physical Biochemistry : (Record no. 7173)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03955cam a22002414a 4500 |
| 001 - CONTROL NUMBER | |
| control field | vtls000003245 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | VRT |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250102223204.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 081220s2000 enka |b 001 0 eng |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 0471986623 (cloth : acidfree paper) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 0471986631 (paper back : acid-free paper) |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] | |
| Level of rules in bibliographic description | 201402040114 |
| Level of effort used to assign nonsubject heading access points | VLOAD |
| Level of effort used to assign subject headings | 201008021408 |
| Level of effort used to assign classification | malmash |
| Level of effort used to assign subject headings | 200812221314 |
| Level of effort used to assign classification | venkatrajand |
| Level of effort used to assign subject headings | 200812201053 |
| Level of effort used to assign classification | Noora |
| -- | 200812201052 |
| -- | Noora |
| 050 00 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QD476.2 |
| Item number | .S42 2000 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Sheehan, David. |
| 9 (RLIN) | 18486 |
| 245 10 - TITLE STATEMENT | |
| Title | Physical Biochemistry : |
| Remainder of title | Principles and Applications / |
| Statement of responsibility, etc. | David Sheehan. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Chichester ; |
| -- | New York : |
| Name of publisher, distributor, etc. | Wiley, |
| Date of publication, distribution, etc. | c2000. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xvi, 349 p. : |
| Other physical details | ill. ; |
| Dimensions | 26 cm. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes bibliographical references and index. |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Preface Acknowledgements Chapter 1 Introduction 1.1 Special chemical requirements of biomolecules 1.2 Factors affecting analyte structure and stability 1.2.1 pH effects 1.2.2 Temperature effects 1.2.3 Effects of solvent polarity 1.3 Buffering systems used in biochemistry 1.3.1 How does a buffer work? 1.3.2 Some common buffers 1.3.3 Additional components often used in buffers 1.4 Quantitation, units and data handling 1.4.1 Units used in this text 1.4.2 Quantitation of protein and biological activity 1.5 Objectives of this book Bibliography Chapter 2 Chromatography 2.1 Principles of chromatography 2.1.1 The partition coefficient 2.1.2 Phase systems used in biochemistry 2.1.3 Liquid chromatography 2.1.4 Gas chromatography 2.2 Performance parameters used in chromatography 2.2.1 Retention 2.2.2 Resolution 2.2.3 Physical basis of peak broadening 2.2.4 Plate height equation 2.2.5 Capacity factor 2.2.5 Peak symmetry 2.2.7 Significance of performance criteria in chromatography 2.3 Chromatography equipment 2.3.1 Outline of standard system used 2.3.2 Components of a chromatography system 2.3.3 Stationary phases used 2.3.4 Elution 2.4 Modes of chromatography 2.4.1 Ion exchange 2.4.2 Gel filtration 2.4.3 Reversed phase 2.4.4 Hydrophobic interaction 2.4.5 Affinity 2.4.6 Immobilised metal affinity chromatography 2.4.7 Hydroxyapatite 2.5 Open-column chromatography 2.5.1 Equipment used 2.5.2 Industrial-scale chromatography of proteins 2.6 High-performance liquid chromatography 2.6.1 Equipment used 2.6.2 Stationary phases in HPLC 2.6.3 Liquid phases in HPLC 2.7 Fast protein liquid chromatography 2.7.1 Equipment used 2.7.2 Comparison with HPLC 2.8 Perfusion chromatography 2.8.1 Theory of perfusion chromatography 2.8.2 The practice of perfusion chromatography 2.9 Membrane-based chromatography systems 2.9.1 Theoretical basis 2.9.2 Applications of membrane-based separations 2.10 Chromatography of a sample protein 2.10.1 Desig |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | An accessible and easy-to-read text for students requiring a broad overview of the key techniques used to characterise the structure and function of complex biomacromolecules such as proteins and DNA. It bridges the gap between general biochemistry textbooks and the more specialist texts covering individual techniques. Topics covered include chromatography, spectroscopy, mass spectrometry, electrophoresis, X-ray diffraction, centrifugation and biocalorimetry. New developments are placed in context by describing the physical principles on which they depend, examining the range of biophysical applications most widely used and, emphasising the overall similarities of experimental approach. Written by a biochemist with extensive teaching experience, the book: describes the advantages and disadvantages of each technique and compares one technique to another; introduces experimental approaches in a non-mathematical way, using practical examples; and provides a bibliography, including useful web sites, at the end of each chapter. This book will be invaluable to undergraduates and postgraduates studying biochemistry, molecular biology and related disciplines. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Physical biochemistry. |
| 9 (RLIN) | 1719 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Library of Congress Classification |
| Suppress in OPAC | No |
| Koha item type | Books |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Library of Congress Classification | Library | Library | First Floor | 21/12/2024 | QD476.2 .S42 2000 | 9786 | 21/12/2024 | 1 | 21/12/2024 | Books |