MARC details
| 000 -LEADER |
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05467cam a22003974a 4500 |
| 001 - CONTROL NUMBER |
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vtls000005170 |
| 003 - CONTROL NUMBER IDENTIFIER |
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VRT |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20250102225214.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
090222s2007 enkaf |b 001 0 eng |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
9780470033562 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
0470033568 |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
|---|
| Level of rules in bibliographic description |
201402040131 |
| Level of effort used to assign nonsubject heading access points |
VLOAD |
| Level of effort used to assign subject headings |
201010090805 |
| Level of effort used to assign classification |
malmash |
| Level of effort used to assign subject headings |
200902220916 |
| Level of effort used to assign classification |
venkatrajand |
| -- |
200902220915 |
| -- |
venkatrajand |
| 050 00 - LIBRARY OF CONGRESS CALL NUMBER |
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| Classification number |
QH588.S83 |
| Item number |
H863 2007 |
| 060 00 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
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| Classification number |
2007 H-427 |
| 060 10 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
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| Classification number |
QU 328 |
| Item number |
H918 2007 |
| 082 00 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
616/.02774 |
| Edition information |
22 |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
Human embryonic stem cells : |
| Remainder of title |
the practical handbook / |
| Statement of responsibility, etc. |
editors, Stephen Sullivan, Chad A. Cowan and Kevin Eggan. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Chichester, West Sussex, England ; |
| -- |
Hoboken, NJ : |
| Name of publisher, distributor, etc. |
John Wiley & Sons, |
| Date of publication, distribution, etc. |
c2007. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xx, 404 p., [4] p. of plates : |
| Other physical details |
ill. (some col.) ; |
| Dimensions |
26 cm. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Includes bibliographical references and index. |
| 505 0# - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
Organization and good aseptic technique in the human embryonic stem cell laboratory / Minal J. Patel, Emma L. Stephenson and Stephen L. Minger -- Sourcing human embryonic stem cell lines / Glyn Stacey -- Culture of human embryos for stem cell derivation / R. Douglas Powers and Jeannine Witmyer -- Derivation of human embryonic stem cell lines / Stephen Sullivan ... [et al.] -- Standard culture of human embryonic stem cells / Jeremy M. Crook, Rachel Horne and Alan Colman -- Chemically-defined culture of human embryonic stem cells / Julie Hsu Clark and Sheng Ding -- Phenotypic analysis of human embryonic stem cells / Jonathan S. Draper, Cheryle A. Séguin and Peter W. Andrews -- Genetic and epigenetic analysis of human embryonic stem cells / Laurie A. Boyer, Rudolf Jaenisch and Maisam Mitalipova -- In vivo characterization of human embryonic stem cells / Scott A. Noggle, Francesca M. Spagnoli and Ali H. Brivanlou -- In vitro differentiation of human embryonic stem cells / A. Henry Sathananthan and Alan Trounson -- Differentiation of human embryonic stem cells into extraembryonic cell types / Cheryle A. Séguin and Jonathan S. Draper -- Directed differentiation of human embryonic stem cells into endoderm / Kenji Osafune ... [et al.] -- Directed differentiation of human embryonic stem cells into mesoderm / Christine Mummery ... [et al.] -- Directed differentiation of human embryonic stem cells into ectoderm / Emily A. Davis ... [et al.] -- Gene targeting in human embryonic stem cells : knock out and knock in by homologous recombination / Thomas P. Zwaka -- RNA interference in human embryonic stem cells / M. William Lensch, Asmin Tulpule and Holm Zaehres -- Generation of human gene reporters using bacterial artificial chromosome recombineering / Andrew J. Washkowitz and David A. Shaywitz. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
With this valuable practical guide, three members of the Harvard Stem Cell Institute have compiled and edited the definite handbook for the exciting new field of human embryonic stem cell research. The editors have gathered protocols from scientists with extensive reputation and expertise, describing and comparing currently used techniques for the culture of human stem cells and discussing the strengths and weaknesses of the different approaches. Human Embryonic Stem Cells: The Practical Handbook contains the first centralised collection of methods used in human embryonic stem cell biology. The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing and characterisation of the cells, and the differentiation of the cells in vitro and in vivo. Lastly, almost all of these protocols can also be used for analyzing and manipulating induced pluripotency iPS stem cells. This allows an even greater number of opportunities for those interested in pursuing work in pluripotent stem cells, disease modelling, and other aspects of basic regenerative medicine research. The novel and useful focus of this book sets it apart from other available books. It compares and evaluates the protocols used in leading laboratories working on human embryonic stem cells. It is centred solely on practical protocols for human (not mouse) embryonic stem cell research. It includes extensive troubleshooting sections. It addresses the different proclivities and behaviours of individual human embryonic cell lines. It contains techniques currently known only to a small number of specialised laboratories worldwide. This handbook represents an essential source of up-to-date practical information for all cell and developmental biologists working with human embryonic stem cells or wishing to enter the field. It is also essential reading for clinical researchers in areas such as diabetes, cardiovascular disease, and neurological diseases. Praise |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Embryonic stem cells |
| General subdivision |
Research. |
| 9 (RLIN) |
54231 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Human embryo |
| General subdivision |
Research. |
| 9 (RLIN) |
54232 |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Embryonic Stem Cells |
| General subdivision |
physiology. |
| 9 (RLIN) |
54233 |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Cell Culture Techniques |
| General subdivision |
methods. |
| 9 (RLIN) |
54234 |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Cell Differentiation |
| General subdivision |
physiology. |
| 9 (RLIN) |
54235 |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Cell Line |
| General subdivision |
physiology. |
| 9 (RLIN) |
54236 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Sullivan, Stephen, |
| Titles and other words associated with a name |
Dr. |
| 9 (RLIN) |
54237 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Cowan, Chad. |
| 9 (RLIN) |
54238 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Eggan, Kevin. |
| 9 (RLIN) |
54239 |
| 856 41 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Materials specified |
Table of contents only |
| Uniform Resource Identifier |
<a href="http://www.loc.gov/catdir/toc/ecip0713/2007011301.html">http://www.loc.gov/catdir/toc/ecip0713/2007011301.html</a> |
| 856 42 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Materials specified |
Contributor biographical information |
| Uniform Resource Identifier |
<a href="http://www.loc.gov/catdir/enhancements/fy0739/2007011301-b.html">http://www.loc.gov/catdir/enhancements/fy0739/2007011301-b.html</a> |
| 856 42 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Materials specified |
Publisher description |
| Uniform Resource Identifier |
<a href="http://www.loc.gov/catdir/enhancements/fy0739/2007011301-d.html">http://www.loc.gov/catdir/enhancements/fy0739/2007011301-d.html</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Library of Congress Classification |
| Suppress in OPAC |
No |
| Koha item type |
Books |
